DNA fRoM dRiEd PlAnT mAtErIaL... A TeDiOuS tAsK tO wOrK oUt !!

Replies to This Discussion

Permalink Reply by Kris Jett on November 23, 2010 at 11:09am

Hi Dhivya,

I don't know if this will help, but the researchers at the University of Johannesburg have shared their plant protocols in a PDF at their site, http://acdb.co.za/. Hope this helps a little!

• ▶ Reply

Permalink Reply by R. Sathishkumar on November 23, 2010 at 11:29am

Thanks Kris Jett for the help by sending the protocol.
Sathish

• ▶ Reply

Permalink Reply by Dhivya on November 26, 2010 at 1:50pm

Hi kris, thank you for sending the protocols :)

• ▶ Reply

Permalink Reply by C. Haris Saslis-Lagoudakis on November 24, 2010 at 11:58am

I have used the protocol that Kris mentions from the UoJ and works very well.
Also, I have used the QIAGEN DNeasy plant mini kit, which also works well (and comes with full protocol). I have managed to get PCR product from a 110 year-old herbarium specimen. The first protocol is better for long term storage (especially when precipitated in iso-propanol) and will probably yield more high molecular weight DNA, so if you are doing AFLPs it's better. But for PCR product, the DNeasy kit will work fine too.
Hint: If you decide to go for the DNEasy, you can reduce the final elution volume at the last step, in order to get more concentrated genomic DNA.
Hope that helps
Haris

• ▶ Reply

Permalink Reply by R. Sathishkumar on November 24, 2010 at 7:42pm

Thank you for the information.

• ▶ Reply

Permalink Reply by Dhivya on November 26, 2010 at 2:01pm

Hi Haris, Thanks a lot for ur information.

i feel u culd help Diego Pignataro (http://connect.barcodeoflife.net/profile/DiegoPignataro) who is in need of sum protocols to extract dna from ancient seeds. The above link helps you to find his home page. Kindly give ur suggestions to him also...

• ▶ Reply