DNA from ancient seeds

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Permalink Reply by Dhivya on November 4, 2010 at 12:15pm

Hi Diego Pignataro,

Im Dhivya.S, Mphil Scholar, Bharathiar University, India. I work to authenticate the Traditional Indian Herbal Medicines, which are composed of dried parts of the plant tissue...i ve send u a pdf file...from tat i ve tried few protocols to extract dna from the dried samples and found to be promising for PCR amplification...i ve also tried PVP, CI-Phenol based methods... Pls do suggest me some protocols whichever u ve tried...

Regards,

Dhivya.S

Attachments:

• DNA Isolation Procedures.pdf, 495 KB

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Permalink Reply by Diego Pignataro on January 18, 2011 at 11:46am

Hi Dhivya,

 

I am really sorry for my delayed answer. I haven´t seen this reply before. Give me your skype account to keep in touch more frequently, I do believe that we have lots of experiences to share.

Mine is: diego.pignataro

 

I am attaching some protocols that I already tested. Good for some plants but for all of them (specially for cinchona sp. that its a Rubiaceae).

 

Cheers,

 

diego

Attachments:

• HERBARIUM SPECIMENS IN DNA PHYLOGENETICS.pdf, 1.9 MB
• piper protocolo de xtraccion.pdf, 386 KB

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Permalink Reply by Isaac Chow on January 18, 2011 at 11:06am

Hi Diego,

 

Me again, we have experience in extracting decades bones for forensic DNA. In this case, CTAB usually perform well over the guanidine hcl. Or simply use QIagen investigator kit. further, try to extract the core part of the seed and at least do some peel off for the outer. remember, extracting DNA for PCR only requires a very very little amount of sample, dont be greedy, quality is the priority.

 

isaac

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Permalink Reply by Diego Pignataro on January 18, 2011 at 11:53am

Hi Isaac,

 

Thanks a lot for your commments and advices. Happy to meet you!. Here is my skype account to keep in touch easily: diego.pignataro (peru)

 

totally right about not to be greedy!

 

 cheers,

 

Diego

 

 

 

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