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Complementary bases represented on Electropherogram

Hi everybody,

I have this set of sequences belonging to a fragment of nuclear DNA. The thing is, a portion of the sequence seems to have the complementary bases also represented. So, at one position where you have an A you also get a “shadowed” T, and where you have a C you also get a “shadowed” G. Has anyone ever encountered this? If so, how do you solve this when you are editing sequences?

Thank you.

Tags: BioEdit, DNA, bases, chromatogram, complementary, editing, electropherogram, sequence

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Permalink Reply by Lee Weigt on August 13, 2012 at 8:56am

can you send the electropherogram so we can see the overall quality, before and after the interesting problem, and the quality scores? does the phenomenon happen or can it be seen in both directions?

thanks Lee (weigtl@si.edu)

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